Micro Chapter 3 Question Preview (ID: 61841)
Microscope And Cells.
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Which microscope achieves the highest magnification and greatest resolution?
a) compound light microscope
b) phase-contrast microscope
c) fluorescence microscope
d) electron microscope
Which of the following correctly traces the path of light through the compound microscope?
a) light source; specimen; condenser; objective lens; ocular lens
b) light source; condenser; objective lens; specimen; ocular lens
c) light source; condenser; specimen; objective lens; ocular lens
d) None of these are correct
You are performing a Gram stain on gram-negative bacteria and you stop after the addition of the mordant. What is the appearance of the bacteria at this point?
a) purple
b) pink
c) colorless
d) None of these
What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?
a) 4.5x
b) 10x
c) 100x
d) 450x
Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are
a) cell walls.
b) endospores
c) capsules
d) flagella
Which step in the Gram stain is the critical step in differentiating gram-positive cells from gram-negative cells?
a) safranin
b) iodine
c) alcohol-acetone
d) crystal violet
Bacterial smears are fixed before staining to
a) make their cell walls permeable.
b) accept the stain.
c) make the cells more visible.
d) affix the cells to the slide.
The purpose of the ocular lens is to
a) magnify the image from the objective lens.
b) decrease the light.
c) increase the light.
d) decrease the refractive index.
Immersion oil is necessary when using the 100x objective on a compound light microscope
a) to lubricate the objective.
b) o prevent the slide from scratching the lens.
c) None of these make sense
d) otherwise too much light is lost to refraction and the image will be fuzzy.
In a completed Gram stain, gram-negative bacteria are colorless.
a) True
b) False
c)
d)
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