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To produce transgenic bacteria that make insulin, which of the following steps did scientists have to do first?
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
Suppose a restriction enzyme recognizes the six-base sequence AAGCTT. Between which two nucleotides would the enzyme have to cut to produce a fragment with sticky ends that are four bases long?
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
A strand of DNA formed by the splicing of DNA from two different species is called
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
What kind of cell or cells were used to make Dolly?
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
One function of gel electrophoresis is to
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
Which of the following is often used as a genetic marker in plasmids?
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
Radioactive or fluorescent-labeled RNA or single-stranded DNA pieces that are complementary to the gene of interest and are used to confirm the presence of a cloned gene are called
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
Plasmids
AA
probes
Use a restriction enzyme to cut out the insulin gene from human DNA.
separate DNA fragments
body cell and egg cell
All of the above
a gene for antibiotic resistance
recombinant DNA
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