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Genetic Engineering

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Instructions: Answer all questions to get your test result.

1) Which of the following is often used as a genetic marker in plasmids?

Aa DNA sequence that serves as a bacterial origin of replication Ba nucleotide labeled with a fluorescent dye Ca gene for antibiotic resistance Da foreign gene

2) To produce transgenic bacteria that make insulin, which of the following steps did scientists have to do first?

AInsert the human insulin gene into a plasmid. BUse a restriction enzyme to cut out the insulin gene from human DNA. CExtract the insulin from the bacterial culture. DTransform bacteria with the recombinant plasmid.

3) What kind of cell or cells were used to make Dolly?

Aegg cell only Bbody cell only Cbody cell and egg cell Degg cell and sperm cell

4) Cloning is a process by which

Amany identical cells may be produced. Ba virus and a bacterium may be fused into one Cmany identical protein fragments are produced. Dundesirable genes may be eliminated.

5) Enzymes that cut DNA molecules at specific places

Awork only on bacterial DNA Balways break the DNA between guanine and adenine Care restriction enzymes Dhave sticky ends

6) Radioactive or fluorescent-labeled RNA or single-stranded DNA pieces that are complementary to the gene of interest and are used to confirm the presence of a cloned gene are called

Aclones Bplasmids Cvaccines Dprobes

7) A strand of DNA formed by the splicing of DNA from two different species is called

Aplasmid DNA Brestriction RNA Crecombinant DNA Ddeterminant RNA

8) Plasmids

AAll of the above Bare circular pieces of bacterial DNA Care often used as vectors in genetic engineering experiments Dare circular pieces of bacterial DNA

9) One function of gel electrophoresis is to

Aseparate DNA fragments Brecombine DNA Cextract DNA Dcut DNA

10) Suppose a restriction enzyme recognizes the six-base sequence AAGCTT. Between which two nucleotides would the enzyme have to cut to produce a fragment with sticky ends that are four bases long?

AGC BAA CCT DAG

*select an answer for all questions

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