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Genetic Engineering
Test Description: Practice
Instructions: Answer all questions to get your test result.
1) Which of the following is often used as a genetic marker in plasmids?
A
a gene for antibiotic resistance
B
a foreign gene
C
a DNA sequence that serves as a bacterial origin of replication
D
a nucleotide labeled with a fluorescent dye
2) To produce transgenic bacteria that make insulin, which of the following steps did scientists have to do first?
A
Extract the insulin from the bacterial culture.
B
Transform bacteria with the recombinant plasmid.
C
Insert the human insulin gene into a plasmid.
D
Use a restriction enzyme to cut out the insulin gene from human DNA.
3) What kind of cell or cells were used to make Dolly?
A
egg cell only
B
egg cell and sperm cell
C
body cell and egg cell
D
body cell only
4) Cloning is a process by which
A
many identical protein fragments are produced.
B
a virus and a bacterium may be fused into one
C
many identical cells may be produced.
D
undesirable genes may be eliminated.
5) Enzymes that cut DNA molecules at specific places
A
are restriction enzymes
B
have sticky ends
C
always break the DNA between guanine and adenine
D
work only on bacterial DNA
6) Radioactive or fluorescent-labeled RNA or single-stranded DNA pieces that are complementary to the gene of interest and are used to confirm the presence of a cloned gene are called
A
clones
B
vaccines
C
plasmids
D
probes
7) A strand of DNA formed by the splicing of DNA from two different species is called
A
recombinant DNA
B
plasmid DNA
C
restriction RNA
D
determinant RNA
8) Plasmids
A
All of the above
B
are circular pieces of bacterial DNA
C
are circular pieces of bacterial DNA
D
are often used as vectors in genetic engineering experiments
9) One function of gel electrophoresis is to
A
separate DNA fragments
B
cut DNA
C
recombine DNA
D
extract DNA
10) Suppose a restriction enzyme recognizes the six-base sequence AAGCTT. Between which two nucleotides would the enzyme have to cut to produce a fragment with sticky ends that are four bases long?
A
AA
B
AG
C
CT
D
GC
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