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Gel Electrophoresis
Test Description: Biology questions about gel electrophoresis
Instructions: Answer all questions to get your test result.
1) In gel electrophoresis, how do we make the DNA migrate through the gel?
A
Large fragments drift to the end of the gel
B
We place a negative electrode away from the wells
C
Gravity
D
We place a positive electrode away from the wells
2) In gel electrophoresis, what does the gel act like?
A
A barrier
B
A gate
C
An open window
D
A filter
3) What do we use to cut the DNA before gel electrophoresis?
A
Lipase
B
DNA polymerase
C
Restriction enzymes
D
Helicase
4) Which is NOT a reason for using gel electrophoresis?
A
Comparing two sets of DNA
B
Organizing DNA in an order that we can see
C
Organizing DNA by the shape of the backbone
D
Organizing DNA fragments from largest to smallest
5) If we have a baby, and we know who the mother is but we want to determine the father using gel electrophoresis, how will we know we have found the father?
A
The father will match all of the DNA bands of the baby
B
The father will match the baby at any band where the baby does not match the mother
C
The father will match the baby at any DNA band where the baby matches the mother
D
The father will not match any DNA bands
6) Which is not true of the DNA analysis used by forensic scientists, (scientists who analyze data to help solve crimes) to identify an individual using DNA left at a crime scene?
A
Forensic scientists use restriction enzymes to cut DNA into pieces.
B
DNA Fingerprinting is different than using fingerprints left by the pattern of skin ridges left by touching an item.
C
Only some bands of the Crime Scene DNA need to match the Suspect DNA to identify the suspect.
D
Gel electrophoresis is used on DNA samples from the crime scene and on DNA samples taken from the suspects.
7) In gel electrophoresis, DNA is separated based on...
A
The length of the restriction site on the DNA strand
B
The size of the DNA fragments
C
The number of species represented in the DNA sample
D
The size of the cell from which the DNA was removed
8) What causes the DNA fragments to move through the gel?
A
The attraction of the DNA to a negative charge.
B
The attraction of the DNA to a neutral charge.
C
The DNA fragment size
D
The attraction of the DNA to a positive charge.
9) What charge does DNA have?
A
positive
B
none
C
negative
D
neutral
10) In gel electrophoresis, the smallest DNA fragments can be found...
A
Stuck in the well
B
Farthest from the well
C
Closest to the well
D
Still in the test tube
*select an answer for all questions
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