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Genetic Engineering
Test Description: Practice
Instructions: Answer all questions to get your test result.
1) Which of the following is often used as a genetic marker in plasmids?
A
a nucleotide labeled with a fluorescent dye
B
a DNA sequence that serves as a bacterial origin of replication
C
a foreign gene
D
a gene for antibiotic resistance
2) To produce transgenic bacteria that make insulin, which of the following steps did scientists have to do first?
A
Insert the human insulin gene into a plasmid.
B
Transform bacteria with the recombinant plasmid.
C
Extract the insulin from the bacterial culture.
D
Use a restriction enzyme to cut out the insulin gene from human DNA.
3) What kind of cell or cells were used to make Dolly?
A
body cell and egg cell
B
egg cell and sperm cell
C
egg cell only
D
body cell only
4) Cloning is a process by which
A
undesirable genes may be eliminated.
B
many identical protein fragments are produced.
C
a virus and a bacterium may be fused into one
D
many identical cells may be produced.
5) Enzymes that cut DNA molecules at specific places
A
are restriction enzymes
B
work only on bacterial DNA
C
have sticky ends
D
always break the DNA between guanine and adenine
6) Radioactive or fluorescent-labeled RNA or single-stranded DNA pieces that are complementary to the gene of interest and are used to confirm the presence of a cloned gene are called
A
plasmids
B
probes
C
clones
D
vaccines
7) A strand of DNA formed by the splicing of DNA from two different species is called
A
recombinant DNA
B
restriction RNA
C
determinant RNA
D
plasmid DNA
8) Plasmids
A
are often used as vectors in genetic engineering experiments
B
are circular pieces of bacterial DNA
C
are circular pieces of bacterial DNA
D
All of the above
9) One function of gel electrophoresis is to
A
separate DNA fragments
B
recombine DNA
C
cut DNA
D
extract DNA
10) Suppose a restriction enzyme recognizes the six-base sequence AAGCTT. Between which two nucleotides would the enzyme have to cut to produce a fragment with sticky ends that are four bases long?
A
GC
B
AA
C
AG
D
CT
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