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PBS 1.2 What Is DNA?
Test Description: Aligned with PLTW 1.2 What is DNA?
Instructions: Answer all questions to get your test result.
1) If a DNA strand has a sequence of GCCATA, what is the sequence of the complimentary strand?
A
TAGGAC
B
CGGTAT
C
CGTAGA
D
GTGATT
2) Which of these bonds are the weakest?
A
bond between adenine and thymine
B
bond between deoxyribose and cytosine
C
bond between guanine and deoxribose
D
bond between phosphate and a deoxyribose sugar
3) What is the charge of DNA?
A
Always neutral
B
Always negative
C
It depends on whose it is
D
Always positive
4) What is Chargaff's rule?
A
Adenine pairs with thymine and guanine with cytosine
B
DNA has the shape of a double helix
C
The backbone of DNA is the same for every living thing
D
All bases are pyrimidines or purines
5) Deoxyribose sugar, phosphate, and a nitrogen base make up a
A
nuclear
B
nucleolus
C
nucleotide
D
nucleus
6) Structurally, which other component is guanine most similar to?
A
Phosphate
B
Adenine
C
Deoxyribose
D
Cytosine
7) You are a DNA analyst. A small sample of DNA was recovered from a crime scene. You need to analyze it. Your first step is to do what?
A
Perform PCR to make copies
B
Run a gel
C
Add restriction enzymes
D
Compare the RFLP's
8) You just added restriction enzymes to the sample. What will the enzymes do to the DNA?
A
Cut it into pieces at specific points
B
Draw the DNA out of the solution
C
Separate the RFLP's
D
Make lots of copies of it
9) The small black dot in a DNA diagram represents
A
thymine
B
deoxyribose
C
phosphate
D
adenine
10) The pentagon in a DNA diagram represents
A
thymine
B
phosphate
C
deoxyribose
D
sugar
11) Thymine always pairs with ____ in DNA.
A
Guanine
B
Thymine
C
Adenine
D
Cytosine
12) Guanine always pairs with ____ in DNA.
A
Guanine
B
Thymine
C
Adenine
D
Cytosine
13) RFLP's move on the gel because of their
A
None of the above
B
Size only
C
Charge only
D
Charge and size
14) The pieces that move farther during electrophoresis move more because they have
A
Fragments don't move
B
Fewer base pairs
C
No base pairs
D
Lots of base pairs
15) Each of the following is an RFLP. Which one would travel the FARTHEST distance on the gel?
A
30 bp
B
90 bp
C
15 bp
D
60 bp
16) Each of the following is an RFLP. Which of the following would travel the SHORTEST distance on the gel?
A
90 bp
B
60 bp
C
15 bp
D
30 bp
17) A restriction enzyme cuts DNA three times. How many fragments are formed?
A
4
B
3
C
5
D
2
18) When looking at the results of the gel electrophoresis, you find that Suspect 6 and the crime scene results have the exact pattern. What does this mean?
A
Suspect 6 is definitely innocent.
B
Suspect 6 was at the crime scene.
C
Suspect 6 is definitely guilty.
D
Suspect 6 was not at the crime scene.
19) When looking at the results of a gel electrophoresis, Suspect 1 and the crime scene have no bands in common. What does this mean?
A
Suspect 1 is definitely innocent.
B
Suspect 1 was not at the crime scene.
C
Suspect 1 is definitely guilty.
D
Suspect 1 was at the crime scene.
*select an answer for all questions
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